Viva Biotech provides customized gene cloning services, including codon optimization, gene synthesis (outsourced) and subsequent cloning. After you provide a gene template, send a target vector and cloning demand, or provide a protein sequence and nucleic acid sequence, we will provide existing or customized services according to the specific specifications required. In addition, we can provide one-stop service for high yield protein expression, purification and production.

· Subclone service

· Site directed mutagenesis cloning service

· Missing, insert mutant cloning service

· Plasmid extraction (Plasmid Endofree Miniprep, Midiprep, Maxiprep, Megaprep, Gigaprep)

· Plasmid endotoxin removal and determination service

Escherichia coli expression

· Large vector and strain collection, specific M9/labeled M9 media

· The constructed plasmid can be transformed into a variety of commercial strains (such as BL21(DE3), BL21 (DE3) Gold, BL21 (DE3) CodonPlus RIPL, and Rosetta)

· Inducible expression at different temperatures (16℃- 37℃) can be performed

· Different culture mediums (LB, TB, 2xYT, M9) can be selected

· Different induction methods (IPTG induction, arabinose induction, autoinduction) can be selected

· Coexpression chaperone can be selected to improve soluble expression

· High throughput expression test based on 96 well plate (which can test the expression differencesof different strains and different constructs)

· Expression of isotope labeled proteins (including 15N labeling, 15N13C double labeling, 15N2Hdouble labeling, 15N13C2H triple labeling, and 13C-Me ILV selective labeling based on 15N2Hdouble labeling)

Baculovirus expression system

· Different vectors/cell lines, membrane protein/protein complexes generation

· Generationand amplification of Baculovirus

· Baculovirus systems: Bac-to-Bac system; Bacmam system; Linearized co-transfection systems such as pVL1392, ProEASY, BD BaculoGold system, pTriEX …

· Optimization of expression: cell lines, infection time, infection ratio (MOI), expression temperature…

· Capacity of large-scale expression:>1000 L/month

· Capacity of construct numbers: >200/month

· Preparation, preservation and optimization expression of BIIC (Baculovirus Infected Insect Cell)

· Insect cell transient experiment (InsectSelect BSD System)

Protein expression with suspension mammalian cells

· Transient transfection expression and BacMam infection expression services in a varietyof suspension cell lines including Expi293F, ExpiCHOS, Freestyle 293F, and Expi293F™ GnTI-cell

· Cell culture service with suspension mammalian cell including transient transfection, viral infection, and large-scale expression with stablecell line culture up to 400 L/month

· Stable cell line generation for protein expression

Yeast expression system

· Production of secreted, intracellular and membrane-bound proteins

· Capacity: >1,000L/year

· Inclusion body purification

Inclusion body/Insoluble protein is expressed, washed followed by denatured and dissolved, and then purified and refolded (or purified after refolding) to obtain soluble protein

· Secretory protein purification

The signal peptide is used to guide the expression of secretory protein outside the cell, and the cell supernatant (culture medium) is collected directly for purification

· Common modifications involved in purification

Biotinylation (in vivo or in vitro), phosphorylation, dephosphorylation, deglycosylation

· Enzyme digestions involved in purification

TEV, Ulp1, PreScission (or 3C), Thrombin, and Ek

· Other technologies involved in purification

Crosslinking, Nanodisc, and Fibrillization

· Production of soluble protein

· Production of renaturation protein

· Production of secretory protein

· Membrane protein assembly