At the biochemical level, we use various methods such as TR-FRET, Alphascreen, FP, and SPR to detect the binary binding of PROTAC and target protein or E3 ligase, as well as the ternary binding of target protein-PROTAC-E3 ligase. At the cellular level, we evaluate the degradation ability of PROTAC on the target protein and the specificity of the pathway. Specific experimental systems are developed for specific target proteins and disease areas to validate the cellular function of PROTAC.
Endogenous Tagging Assay
